PRODUCT: DRAQ7 Application Area: Flow Cytometry Ease of Use: ⭐⭐⭐⭐ After Sales Service: Not required Value for Money: ⭐⭐⭐⭐Average rating: 4.0 Comments: To test the effects APPA (10 µg/ml), AP (2.3 and 10 µg/ml) and […]
Ferroptosis has become a promising target for cancer therapy. This regulated non-apoptotic cell death process is described as an iron-dependent lipid peroxidation, specifically necessitating peroxidation of polyunsaturated fatty acid-containing phospholipids. The protective mechanism in healthy cells is for the resulting lipid hydroperoxides to be converted to the corresponding lipid alcohols by glutathione peroxidase 4 (GPX4) […]
PRODUCT: DRAQ5 Application Area: Cell Sorting Ease of Use: ⭐⭐⭐⭐⭐ After Sales Service: ⭐⭐⭐⭐ Value for Money: ⭐⭐⭐⭐⭐Average rating: 4.7 Comments: We used Invitrogen™ eBioscience™ DRAQ5™ primarily as a far-red nuclear stain for both flow […]
Features with MDS need to be better defined to be robust indicators of disease. Disease experts from Lund University and Skåne Regional Laboratories show that Imaging Flow Cytometry (IFC) can provide the statistical discrimination of cellular features that are cellular hallmarks of MDS. Notably they used DRAQ5 to trigger cellular events, widely used elsewhere. However, […]
Two new papers utilizing DRAQ5 to facilitate high quality cell sorting to downstream single cell RNA sequencing to unpick the molecular backdrop to atherosclerotic plaque cell biology. In the first of these, from Muredach Reilly’s lab at Columbia University, fibroblasts were profiled. Four distinct subpopulations were identified – all of which diminished in number upon […]
PRODUCT: DRAQ5 Application Area: Immunofluorescence microscopy Ease of Use: ⭐⭐⭐⭐⭐ After Sales Service: ⭐⭐⭐⭐ Value for Money: ⭐⭐⭐⭐⭐Average rating: 4.7 Comments: DraQ5 was used in a 1:1000 dilution for immunofluorescence staining of murine megakaryocytes to highlight […]
PRODUCT: DRAQ5 Application Area: Immunofluorescence microscopy Ease of Use: ⭐⭐⭐⭐⭐ After Sales Service: ⭐⭐⭐⭐⭐ Value for Money: ⭐⭐⭐⭐Average rating: 4.7 Comments: DRAQ5 was used in confocal microscopy. The dye worked well. The results were of high quality. […]
It has proved difficult to predict the likelihood of progression of disease in MS. New work shows that there is a complex relationship between ependymal cells at the tissue interface with CSF and extrinsic factors and also with cell-intrinsic factors that provide new avenues for prognostic and perhaps therapeutic advantage. A team led by Dr […]
A team lead by scientists at Thomas Jefferson University have demonstrated a new opportunity for intervening in ALS/FTD. They worked on the hypothesis that aberrant arginine-rich dipeptide repeat (R-DPR) proteins are a key toxicity, aggregating with RNA binding proteins. They demonstrated that it was possible to disrupt the aggregation, though initially at the cost of […]
Endothelial to Mesenchymal Transition (EndMT) is a well-understood in development but not in the adult. This may be important in development of disease or progression through invasion, as in metastasis. A laboratory at the University of Binghamton, NY has developed a 3D fluidic device that permits the application of shear stress to endothelial cells to […]