3D microtissues are now well-established as an improved in vitro recapitulation of tumor and the likely response or resistance to therapeutic interventions. To that end, a lab at the University of Saskatchewan, Saskatoon, has developed a model micro-tissue platform for triple-negative breast cancer cell lines (TNBC) and having the feature that these microtissues are free-floating in a […]
Fascinating new research headed by a team in Utrecht has applied the strategies now aiding drug discovery to inform and direct clinical investigations of variants of unknown significance (VUS) that are a product of the genetic revolution in medical diagnosis. With the emergence of VUS that result from their detection by DNA sequencing but which […]
This new work builds on earlier studies to determine a best methodology to clear and image tumour spheroids (Nürnberg et al. 2020) previously reviewed here. This showed the performance advantage of a low-cost glycerol-based RI correction and nucleus counterstaining with DRAQ5. The authors, from the laboratory of Rüdiger Rudolph (Mannheim Univ. of Applied Sciences, DE), described […]
PRODUCT: DRAQ7 Application Area: High Content / Phenotypic Screening Ease of Use: ⭐⭐⭐⭐⭐ After Sales Service: Not required Value for Money: ⭐⭐⭐⭐Average rating: 4.5 Comments: I use DRAQ7 to monitor cell death by high […]
PRODUCT: DRAQ5 Application Area: Cell Sorting Ease of Use: ⭐⭐⭐⭐⭐ After Sales Service: Not required Value for Money: ⭐⭐⭐⭐⭐Average rating: 5.0 Comments: Name: Shirin Schneeberger Organisation: Charite, Germany
PRODUCT: DRAQ5 Application Area: Immunofluorescence microscopy Ease of Use: ⭐⭐⭐⭐⭐ After Sales Service: ⭐⭐⭐⭐⭐ Value for Money: ⭐⭐⭐Average rating: 4.3 Comments: To our experience, the best staining was achieved when dye Draq5 was added to cells […]
Led by Prof Efstathios Karathanasis, a group at Case Western Reserve University has developed an improved and tunable technology for the delivery of gene silencing signals (siRNA for example) to achieve PD-L1 gene silencing. The vehicle lipid nanoparticles (LNPs) have modified PEG content that adjusts their cellular uptake. In one series of experiments to show the […]
PRODUCT: DRAQ5 Application Area: Confocal Imaging Ease of Use: ⭐⭐⭐⭐⭐ After Sales Service: ⭐⭐⭐⭐ Value for Money: ⭐⭐⭐⭐Average rating: 4.3 Comments: DRAQ5 was used for nucleus staining in our study. Performance: Great Easy to handle […]
PRODUCT: CyTRAK Orange Application Area: High Content / Phenotypic Screening Ease of Use: ⭐⭐⭐⭐⭐ After Sales Service: ⭐⭐⭐⭐ Value for Money: ⭐⭐⭐⭐⭐Average rating: 4.7 Comments: We used CyTRAK Orange to stain cultured cells in […]
The need for a reliable supply of O-negative (universal donor) red blood cells has driven ex vivo RBC manufacture closer to reality in recent years. However, the necessity for erythropoietin (EPO) in the cell culture to promote lineage maturation has remained a significant cost burden. New work from Stanford and UCSF aims to overcome this. […]