HypoxiTRAK™
HypoxiTRAK™ is a novel, far-red fluorescing dye that reveals the hypoxic experience of individual cells. It is non-toxic to normoxic cells. HypoxiTRAK™ enables entirely new assay approaches to functional hypoxia.
HypoxiTRAK™ can be used in flow cytometry and fluorescence imaging and is spectrally compatible with common visible range fluors including FITC and R-PE.
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HypoxiTRAK™
Technical Information
- HypoxiTRAK™ is supplied as a faint blue aqueous solution at a concentration of 10μM.
- Products are shipped at ambient temperature, but on receipt packs should be stored at 2-8°C. Do NOT freeze! HypoxiTRAK™ can come out of solution when frozen and it is difficult (but not impossible) to get it back into solution.
- HypoxiTRAK™ can be diluted in culture media (e.g. RPMI 1640) and physiological buffers (eg PBS, Hanks’s, etc.).
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The far red fluorescent HypoxiTRAK™ molecular probe is designed to convert exclusively in hypoxic cells to a bioactive metabolite that retains the parent molecule fluorophore but which now accumulates in cells to provide a positive and persistent far red fluorescent signature.
- HypoxiTRAK™ is a member of the anthraquinone family and shares spectral characteristics with the related cell permeant DNA dye DRAQ5™.
- HypoxiTRAK™ is designed to activate by bioreduction at biologically relevant levels of hypoxic environments (e.g. tumour cores) compared to normal tissues.
- The bioactive metabolite accumulates in cells, proportional to the hypoxia experienced, providing a persistent far-red fluorescent signature and induces cell arrest thereby marking and ‘freezing’ hypoxic cells, enabling a direct read-out of an hypoxic cell fraction.
The hypoxia sensing range for HypoxiTRAK™ is relevant to biomarker and hypoxia-targeting drug development:
- HypoxiTRAK™ is ideal for studies over several days.
- HypoxiTRAK™ read-out is direct with no requirement for cell processing.
As no fixation is required, HypoxiTRAK™ can provide a convenient negative selection for cells not experiencing significant hypoxia within heterogeneous populations, including 3D culture.
HypoxiTRAK™ shows low perturbation in the absence of bioactivation while the unique far-red fluorescence signature offers compatibility with other end-point assays for hypoxia that use fluorescence signatures.
in FLOW CYTOMETRY:
- extract statistical information on the distribution of hypoxic experience over both time and treatment conditions
in FLUORESCENCE MICROSCOPY:
- gain understanding of the spatial hypoxic experience in micro-tissues and spheroids
What our Customers are saying about HypoxiTRAK™:-
- HypoxiTRAK™ is a NEW product from BioStatus. Be one of the first to review HypoxiTRAK™ and publish your experience here.