PRODUCT: DRAQ5 Application Area: Cell counting in high-content compound screening Ease of Use: ⭐⭐⭐⭐⭐After Sales Service: ⭐⭐⭐⭐⭐ Value for Money: ⭐⭐⭐⭐⭐Average rating: 5.0 Comments: We have run high-content compound screening and used this product for cell […]
PRODUCT: DRAQ5 Application Area: Mark cellular nucleus Ease of Use: ⭐⭐⭐⭐⭐After Sales Service: ⭐⭐⭐⭐ Value for Money: ⭐⭐⭐⭐Average rating: 4.3 Comments: I acquired high-quality results. Name: Francesco Bellanti Organisation: University of Foggia
PRODUCT: DRAQ5 Application Area: Immunocytochemical analysis Ease of Use: ⭐⭐⭐⭐⭐After Sales Service: ⭐⭐⭐⭐ Value for Money: ⭐⭐⭐⭐Average rating: 4.3 Comments: This product is very easy to use and can stain nuclei with high sensitivity. Name: N/A Organisation: N/A, […]
DRAQ™ dyes have become a standard tool in FACS sample preparation for downstream molecular analysis (e.g. snATAC, GWAS, RNAseq, etc.) Here are recent references to DRAQ7™: Single cell suspensions were generated from fresh prostate tissue and prepared for BD Biosciences Rhapsody analysis with Calcein AM and DRAQ7™ used to evaluate dead/damaged cell numbers prior to the […]
The earlier demonstrations of the capability of the imaging flow cytometer to provide an unattended, automated analysis for chemical genotoxicity or radiation exposure has now been supported by an inter-laboratory comparison study by scientists at GSK, Broad Institute and Swansea, Cardiff, Newcastle, and Cambridge Universities. They demonstrated that the procedures on the Amnis Imagestream, which included DRAQ5 […]
The powerful technique “CODEX” recently developed by the Stanford University laboratory of Garry Nolan enables multiplex imaging (and therefore deep phenotyping) of single biological samples of solid tissue, using iterative cycles of labeling compatible with a standard fluorescence microscope. In two recent examples the inventing laboratory has utilised DRAQ5 as the nuclear counterstain, detected in […]
It is well understood that small molecule drugs are bound by plasma proteins. This plasma protein binding (PPB) varies dramatically from compound to compound and is determined indirectly as a ‘PPB adjusted’ IC₅₀. Despite the inaccuracies of this PPB adjusted IC₅₀ the alternative gold standard of in vivo measurement of free drug is complex and therefore restricted […]
To better understand cell biology in a more physiologically-relevant context we have progressively moved to the use of 3D cell culture in place of 2D adherent cells on plastic. These spheroids and microtissues (e.g. organoids) can be derived from single cell lines, progenitor cells, or increasingly also co- and tri-cultures to further increase relevance. This […]
In a comprehensive study to evaluate the properties of transplantable patient-derived gut organoids scientists at The Francis Crick Institute, Great Ormond Street Hospital and UCL used a suite of analyses, ranging from metabolic, biochemical and multi-lengthscale and multi-feature imaging techniques. In one iteration, they combined µCT and TEM/SBF-SEM/SEM montage. µCT was used to check the […]
PRODUCT: CyTRAK OrangeApplication Area: 2-photon live-imaging Ease of Use: ⭐⭐⭐⭐⭐After Sales Service: ⭐⭐⭐⭐ Value for Money: ⭐⭐⭐⭐Average rating: 4.3 Comments: We used CyTRAK Orange to label nuclei of acute brain slices […]