A new review article published in the September issue of Clinical Cytometry by scientists from Advanced Therapeutics at the Scottish National Blood Transfusion Service highlights the central importance of flow cytometry in the development and qualification of advanced cell therapeutics. The authors emphasise focusing on good practice and then the identity, stability and function of […]
Scientists from Wageningen University and Friesland Campina have investigated the immuno-modulatory properties of bovine lactoferrin (LF), an abundant glycoprotein of bovine milk. Overall, they conclude that LF can play a role in immune homeostasis in the human GI tract, based on the findings that LF modifies dendritic cell (DC) differentiation and reduces the response by […]
PRODUCT: DRAQ5 Application Area: Analyzed nuclei in Drosophila ovaries using Draq5 Ease of Use: ⭐⭐⭐⭐⭐After Sales Service: ⭐⭐⭐⭐ Value for Money: ⭐⭐⭐ Average rating: 4.0 Comments: The product itself is easy to use but the results are not […]
PRODUCT: DRAQ5 Application Area: Highlight nuclei in mitosis imaging Ease of Use: ⭐⭐⭐⭐⭐After Sales Service: ⭐⭐⭐⭐⭐ Value for Money: ⭐⭐⭐Average rating: 4.3 Comments: The product is very easy to use and I followed the manufacturer’s instructions. […]
A robust and simple methodology for seven-colour fluorescence microscopy has recently been presented in a technical note. Interestingly, this has been achieved using a widely available and standard widefield microscope with a mercury lamp as excitation source. As such, this technique should be within the reach of any pathology laboratory, including in the developing nations. […]
PRODUCT: DRAQ5 Application Area: immunofluorescence and confocal microscopy Ease of Use: ⭐⭐⭐⭐After Sales Service: ⭐⭐⭐⭐⭐ Value for Money: ⭐⭐⭐⭐Average rating: 4.3 Comments: Very useful as nuclear marker in well-preserved tissue sections and in cell cultures. Not suitable […]
The lab of Prof. Jochen Herms (LMU-Munich) has developed a simplified CLEM sample-processing workflow to allow study of a single region of interest (ROI) – the tripartite synapse – from in vivo 2-P microscopy through to FIB/SEM. The alignment and registration of the tissue section through the various stages was achieved by slide mounting and […]
Scientists led by Assoc. Prof. Anirban Banerjee, IIT, Mumbai have made a significant and deep investigation into infection by Streptococcus pneumoniae where tissue-destination and persistence are highly dependent upon the expression level of the major virulence factor pneumolysin (Ply), a pore-forming toxin. Even in an isogenic culture Ply was shown to be expressed over […]
PRODUCT: DRAQ5 Application Area: bacterial pathogenesis and host-pathogen interaction Ease of Use: ⭐⭐⭐⭐⭐After Sales Service: ⭐⭐⭐⭐ Value for Money: ⭐⭐⭐⭐Average rating: 4.3 Comments: We used DRAQ5 for labeling our bacteria of interest for live imaging the […]
Since the development of Light Sheet Microscopy by the laboratory of Prof. Ernst Stelzer more than 10 years ago there have been many publications utilising DRAQ5™ and DRAQ7™. A selection of these are presented below with brief notes on the samples that were interrogated. Part of the power of Light Sheet Microscopy is the ability […]