A new application note authored by Brad Larson, Principal Scientist at Biotek Instruments, Inc. showcases their integrated BioSpa platform, which includes the Cytation™5, and the combination of three fluorescent probes to unpick and quantify mechanisms of cell death. The methodologies described should see wide applicability for the investigation of unwanted drug toxicity, cancer chemotherapeutic screening, […]
DRAQ7™ has become the choice for image-based real-time / time-lapse cell health monitoring. The following papers add to the evidence base on the product’s broad applicability and high performance: Spheroid / 3D Culture Cell Health Analysis: Dunlop, E. A., Johnson, C. E., Wiltshire, M., Errington, R. J., & Tee, A. R. (2017). Targeting protein […]
Researchers’ confidence in DRAQ7™ as the viability probe for single cell sorting shown in a growing citation list. Since we published our white paper on single cell sorting (available here) in Spring 2018 there have been several more articles that we believe merit attention.. 1. Sorting for molecular analysis e.g. RNA-seq 2. Sorting for downstream cell culture […]
The CryoChem Method (CCM) is a new hybrid approach to the processing of cryofixed cells and tissues that enables preservation of ultrastructural morphology while being compatible with genetic EM tags, fluorescent proteins and counterstaining, and onwards to SBEM and CLEM. The research, led by Professor Mark Ellisman, UCSD, benchmarks this new approach in a variety […]
Fascinating new research led by scientists at the MRC Toxicology Unit (Leicester, UK) explores a key and non-canonical role for Drosha in the DNA Damage Response (DDR). In wide-ranging studies Lu et al. show the dependence of DDR early in the response, upstream of the decision to repair double-strand breaks (DSB) using homologous recombination (HR) […]
PRODUCT: DRAQ5 Application Area: Airway cell nuclei stain Ease of Use: ⭐⭐⭐⭐⭐After Sales Service: ⭐⭐⭐⭐⭐ Value for Money: ⭐⭐⭐⭐⭐Average rating: 5.0 Comments: Draq5 is an easy to use and affordable live cell stain. Name: Derek McMahon Organisation: Univ of […]
Around the world, scientists just like you have pondered how to preserve the signal from their GFP expressing cells after having watched weeks of work disappear before their very eyes.. Fixation procedures can allow leakage out of the GFP-tagged protein or be directly detrimental to the fragile amino acid cage that enables GFP’s remarkable properties, properties that […]
Are you using the Imagestream®x (ISX) imaging flow cytometer from Merck? If you are, then you will probably have used DRAQ5™ at some point in your research work! There are more than 300 publications citing use of DRAQ5™ on Imagestream 100 and x platforms. Why..? DRAQ5™ gives clean nuclear labeling – enabling compartment segmentation, morphometrics, study […]
PRODUCT: DRAQ7 Application Area: Quantitative measurement of dead cells in live cell imaging Ease of Use: ⭐⭐⭐⭐⭐After Sales Service: ⭐⭐⭐⭐ Value for Money: ⭐⭐⭐⭐⭐Average rating: 4.7 Comments: DRAQ7 works perfectly and always stain the nucleus of […]
PRODUCT: DRAQ7 Application Area: Analyze number of dead cells at each timepoint Ease of Use: ⭐⭐⭐⭐⭐After Sales Service: ⭐⭐⭐⭐ Value for Money: ⭐⭐⭐⭐Average rating: 4.3 Comments: My colleagues and I could see a […]