Endothelial to Mesenchymal Transition (EndMT) is a well-understood in development but not in the adult. This may be important in development of disease or progression through invasion, as in metastasis. A laboratory at the University of Binghamton, NY has developed a 3D fluidic device that permits the application of shear stress to endothelial cells to […]
3D microtissues are now well-established as an improved in vitro recapitulation of tumor and the likely response or resistance to therapeutic interventions. To that end, a lab at the University of Saskatchewan, Saskatoon, has developed a model micro-tissue platform for triple-negative breast cancer cell lines (TNBC) and having the feature that these microtissues are free-floating in a […]
Fascinating new research headed by a team in Utrecht has applied the strategies now aiding drug discovery to inform and direct clinical investigations of variants of unknown significance (VUS) that are a product of the genetic revolution in medical diagnosis. With the emergence of VUS that result from their detection by DNA sequencing but which […]
This new work builds on earlier studies to determine a best methodology to clear and image tumour spheroids (Nürnberg et al. 2020) previously reviewed here. This showed the performance advantage of a low-cost glycerol-based RI correction and nucleus counterstaining with DRAQ5. The authors, from the laboratory of Rüdiger Rudolph (Mannheim Univ. of Applied Sciences, DE), described […]
Led by Prof Efstathios Karathanasis, a group at Case Western Reserve University has developed an improved and tunable technology for the delivery of gene silencing signals (siRNA for example) to achieve PD-L1 gene silencing. The vehicle lipid nanoparticles (LNPs) have modified PEG content that adjusts their cellular uptake. In one series of experiments to show the […]
The need for a reliable supply of O-negative (universal donor) red blood cells has driven ex vivo RBC manufacture closer to reality in recent years. However, the necessity for erythropoietin (EPO) in the cell culture to promote lineage maturation has remained a significant cost burden. New work from Stanford and UCSF aims to overcome this. […]
New research published in Nature Communications offers an exciting new cell therapy option using allogeneic macrophage drug conjugates (MDC) to better attack solid tumors, in an effort to overcome some of the limitations experienced by other cell-based therapies. The work was led by scientists at Cellis AG, a European biotechnology company with focus in cell-based oncology therapy […]
In this new work to determine the role of purines in innate immunity a group led by scientists at UCSD used C. elegans orthologs of enzymes in the human purine salvage pathway. In one aspect of this work they also treated HUVECs with concentrations of deoxyadenosine with and without anti-IFN antibody and then infected the […]
Fascinating new knowledge on hypotonic vacuoles from a collaborative effort between Kent State Univ, Hamburg and Salzburg. Despite the well-described and long-understood rationale for vacuoles in lower order eukaryotes little work has been done to determine the conditions for and source of vacuoles in mammalian cell systems. This new work published in Scientific Reports and […]
PRODUCT: DRAQ7 Application Area: Biochemistry Ease of Use: ⭐⭐⭐⭐⭐ (5.0) After Sales Service: ⭐⭐⭐⭐⭐ (5.0) Value for Money: ⭐⭐⭐⭐ (4.0) Average Rating: 4.7 Comments: Very good and easy to work with. We use it in almost all projects in our lab. Name: Rodrigo Oliveira Organisation: Universidade de São Paulo, Faculdade de Odontologia de Bauru Review […]