CyTRAK Orange™

The Science

• CyTRAK Orange™ can be used as a LIVE or fixed cell counterstain or as a cell location dye to define the perimeter of the cell in a variety of cell-based assays. It can be excited at 488nm and 534nm with optimal emission at 615nm.



• It can be used in flow cytometry to separate (gate) nucleated cells without the time-consuming and potentially damaging RBC lysis procedures.
• It is highly compatible with existing protocols and proven across a wide range of instrumentation platforms: flow cytometers, epifluorescence and confocal microscopes and image-based HCS platforms.

Why CyTRAK Orange™?

• CyTRAK Orange™ co-excites at 488 nm with eGFP, FITC and Cy2 flurophores avoiding the need for laser alignment in confocal instruments when this combination is used.
• CyTRAK Orange™ has an emission profile that permits the simultaneous acquisition of commonly used "green" fluorphore signals (e.g. eGFP, FITC and Cy2) AND far-red FRET dyes (e.g. PE- and APC- Cy7) combined with discrimination of both the nuclear and cytoplasmic compartments.
• A separate series of parameters can be detected with red-exciting fluorphores such as APC, APC-Cy7 since CyTRAK Orange™ is not excited at 633 or 647 nm.
• Wide platform compatibility - blue/green excited (Exmax 510 nm)
• GFP/FITC compatible - orange emission (Emmax 615 nm)
• Multi-colour compatible - no UV /red excitation
• Compartments: 1 dye - differential nucl:cyto staining
• Demarks cell boundaries - even staining of the cytoplasm
• Allows assay development - works in live or fixed cells
• Time-saving - rapid staining, no-lyse/no-wash protocols
• Convenient - ready-to-use and buffer compatible
• Reliable - low photobleaching and chemically stable

Technical Information

CyTRAK Orange™ is a novel fluorescent dye that preferentially stains the nucleus, but also defines the cytoplasmic area in both LIVE and fixed preparations.

This dual compartment label provides rapid, one-step cell feature discrimination, creating new opportunities for cell-based assays where the cell location, cell perimeter, cell shape and cell spread parameters define the assay at the single cell level.

• CyTRAK Orange™ does not crosslink with or esterify cytoplasmic proteins.
• CyTRAK Orange™ does the work of two fluorophores. This leaves more opportunity and choice in designing the assay for the cells of interest.
• Products are shipped at ambient temperature, but on receipt packs should be stored at 2-8°C. Do NOT freeze! CyTRAK Orange™ can come out of solution when frozen and it is difficult (but not impossible) to get it back into solution.

You can find much more technical information in the folders below:-