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What our customers are saying about CyGEL™
The protozoan parasites Leishmania major and Trypanosoma brucei are causative agents of leishmaniasis and African trypanosomiasis, respectively. Live imaging of these organisms is hampered by the rapid flagellum-driven motility of these species.We found that CyGEL was suitable for complete immobilisation of procyclic stage L. major and T. brucei for 90 minutes without affecting cell viability. This has enabled us to analyse a GFP-tagged form of the L. major membrane-associated protein, HASPB, using live confocal imaging and FRAP. (Price et al, 2010, Molec Biochem Parasitol)
Helen Price, PhD, Keele University, UK
The Science
CyGEL™ is a thermo-reversible hydrogel. Unusually, it is a liquid when cooled and gel when warm. It is compatible with (and non-toxic to) cells, tissues and model organisms. CyGEL™ has been formulated to transition from liquid to gel at ca. 21 °C (i.e. room temperature) allowing immobilization of non-adherent (suspension) cells, beads, micro-tissues, parasites, worms and embryos (such as drosophila), which can be recovered non-destructively by cooling and/or over-dilution using cold buffer.
- CyGEL™ is optically clear, inert and non-quenching. It has no visible-range autofluorescence and a refractive index (R≃1.37) equivalent to that of water.
- CyGEL™ is designed as convenient imaging mountant and not as a growth matrix.
Why CyGEL™?
There are many reasons why CyGEL™ will help your research:-
IT’S EASY - ready-to-use, straight from the fridge – no powders to dissolve! IT’S GENTLE - immobilize your living cells or organisms for microscopy IT’S CLEAR - optically, it’s just like water, with no autofluorescence IT’S REVERSIBLE - image your precious sample and get it back alive!
CyGEL™ can bring significant benefits to your application:
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in MICROPLATE CYTOMETRY:
- Immobilize non-adherent and motile objects for 2-D and cytometric analysis in HIGH CONTENT SCREENING:
- Immobilization of non-adherent and fragile 3-D structures in microplates in FLUORESENCE MICROSCOPY:
- Take composite images of motile organisms with confidence and control in IN VITRO TOXICOLOGY:
- Monitor cellular events and organism response in real-time and 3D in APOPTOSIS:
- Observe live non-adherent cells with in-gel probes
Technical Information
CyGEL™ is supplied as an aqueous solution with 40X PBS. Products are shipped at ambient temperature, but on receipt packs should be stored at 2-8 °C. Do NOT freeze! Freezing CyGEL™ may affect its performance.
CYGEL™ can be diluted 10 - 20% v/v with buffers and culture media containing cells, tissues or organisms. CyGEL™ will accept small molecules such as viability and viability dyes and anaesthetics.
You can find much more technical information in the folders below:-
KEY INFORMATION
You can view, share and download the information below by clicking on the links:-
CyGEL™ Technical Data Sheet (PDF)
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KEY REFERENCES
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Search Online: There are many independent references for CyGEL™ online. We have listed some popular papers in the link below for your convenience:
Alternatively you can use the Google Scholar facility below to find exactly what you are looking for. Just type other keywords alongside the product name - such as cell type, instrumentation, research area - in the search box below and Google Scholar will find all of the relevant references online! |
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SAFETY DATA SHEET
You can view, share and download a country specific Safety Data Sheet (PDF) in your language by clicking the relevant flag below:-
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CUSTOMER REVIEWS
What our Customers are saying about CyGEL™:-
- "The protozoan parasites Leishmania major and Trypanosoma brucei are causative agents of leishmaniasis and African trypanosomiasis, respectively. Live imaging of these organisms is hampered by the rapid flagellum-driven motility of these species.
We found that CyGEL™ was suitable for complete immobilisation of procyclic stage L. major and T. brucei for 90 minutes without affecting cell viability. This has enabled us to analyse a GFP-tagged form of the L. major membrane-associated protein, HASPB, using live confocal imaging and FRAP. (Price et al, 2010, Molec Biochem Parasitol) ”
Helen Price, PhD, Keele University, UK - "Under auspices of the ABRF (Association of Biomolecular Resource Facilities) there was a need to create a microscope “test” sample for imaging beyond the coverslip (100µM in Z). To accomplish this, a mounting media with a refractive index approximately that of water was needed to suspend fluorescent beads.
CyGEL™ provided a practical and cost effective solution, with excellent stability. This thermoreversible gel with its reversed viscosity/temperature properties allowed a high concentration of beads to be injected into a predetermined location with little diffusion.”
Richard Cole, PhD, SUNY, USA