DRAQ7™
DRAQ7™ is a new far-red fluorescent DNA dye that ONLY stains the nuclei in DEAD and permeabilized cells.
DRAQ7™ has many applications in imaging, cytometry and screening and is highly compatible with existing protocols across a wide range of instrumentation platforms.
DRAQ7™ is available in one concentration: 0.3mM. The 0.3mM Packs come in two sizes; 250μl and 1ml. Our volume pack of 5x 1ml provides best value for routine use. Please get in touch if you would like to order bulk volumes of DRAQ7™. You can order DRAQ7™ directly from this website – see below:
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DRAQ7 Starter Pack
Format
- 1 × 250 µl
Typical output*
- 50 Flow Cytometry Assays
- 250 Cell Health Assays
DRAQ7 Regular Pack
Format
- 1 × 1 ml
Typical output*
- 200 Flow Cytometry Assays
- 1000 Cell Health Assays
DRAQ7 Volume Pack
Format
- 5 × 1 ml
Typical output*
- 5 × 200 Flow Cytometry Assays
- 5 × 1000 Cell Health Assays
Technical Information
- DRAQ7™ is supplied as a blue aqueous solution in two concentrations: 0.3mM and 1.0mM.
- Products are shipped at ambient temperature, but on receipt packs should be stored at 2-8°C. Do NOT freeze!
- DRAQ7™ can be diluted in culture media (e.g. RPMI 1640) and physiological buffers (eg PBS, Hanks’s, etc.) and mixed with fixatives such as formaldehyde.
You can view, share and download the information below by clicking on the links:-
There are many useful independent references for DRAQ7™ online. We have listed some popular papers in the link below for your convenience:
Alternatively use our handy Google Scholar Link below then type keywords alongside the product name to find specific references – e.g cell type, instrument, research focus
You can view, share and download a country specific Safety Data Sheet (PDF) in your language by clicking the relevant flag below:-
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- DRAQ7™ is a small molecule closely related to DRAQ5™. It has peak absorbances at 600nm and 646nm. It fluoresces in the far-red / near infra-red (NIR) peaking at 697nm (bound to dsDNA). On a flow cytometer it is possible to detect DRAQ7™ using blue laser (488nm) excitation.
Multi-wavelength imaging with UV / vis fluorochromes:
- NO fluorescence enhancement upon DNA binding
- low photobleaching effect
- compatible with optics of flow, laser scanning cytometers and confocal and lamp-based fluorescence microscopes
DRAQ7™ is an ideal far-red DNA counterstain for IF/IHC, high content screening and cell-based assays. DRAQ7™ has many applications and is highly compatible with existing protocols across a wide range of instrumentation platforms. Key benefits include:-
- does NOT enter intact, live cells
- it is excited by blue through red lasers
- DRAQ7™ is NON-TOXIC in long-term culture
- an ideal replacement for propidium iodide (and 7-AAD) having far better spectral properties: no UV excitation and no emission overlap with PE and homologues
- allows a new design of combination analysis for cell health and status
- viability/integrity can be directly interchanged with DRAQ5™ (DNA content)
- rapid staining of dsDNA/nuclei of DEAD or permeabilised cells
- combination with live cell dyes for dead/live discrimination
- easy to use – no wash, no RNase needed
- ideal for use with GFP & FITC labels – DRAQ7™ fluoresces in the far-red region
- Cost-effective analysis
What our Customers are saying about DRAQ7™:-
- “This has enabled us to do experiments that were not otherwise possible before. Not only is it a much cleaner signal than other viability dyes, but the fact that it is non-toxic has enabled us to do time courses with the same samples. We have performed 3D spheroid experiments over a 4-week period, reading plates every 72 hours with zero toxicity! It therefore saves us large amounts of money that would have been spent on ending experiments at each time point using toxic dyes. Highly recommended!
Dr. Gareth Griffiths, Imagen Biotech