DRAQ7™ permits a new kinetic
understanding of SP analysis.
- Membrane-impermeant DRAQ7™
allows removal of interfering Hoechst signals coming from dead and damaged
cells. - DRAQ7™ is non-toxic,
does not passively enter intact cells during the extended exposure times
required and also allows sorting of the viable SP sub-populations. - DRAQ7™ has no
UV-excitation, avoiding PI’s red emission overlap. - Far-red DRAQ7™
permits multi-colour phenotypic SP analysis.
REFERENCE:
Smith, P.J. et al. Kinetic analysis of
intracellular Hoechst 33342-DNA interactions by flow cytometry: Misinterpretation
of side population status?
Cytometry Part A. Early View – published
online November 7, 2012 DOI: 10.1002/cyto.a.22224
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