CyTRAK Orange has been applied for “live gating” in flow cytometric analysis of complex samples.
See:
Mathis et al. doi:10.1038/leu.2013.178
&
Geis et al. DOI: 10.1111/boc.201400079
.. for examples of multi-colour protocols using CYTRAK Orange to positively select intact cells from debris or necrotic/apoptotic cells.
This adds to the well-tested option of the related far-red DRAQ5 gating methodology.