In-Vitro Toxicology
In vitro toxicity testing studies the effects of toxic chemical substances on cultured cells or micro-tissues. It is used primarily to identify potentially hazardous chemicals in the early stages of development of new pharmaceuticals or agrichemicals.
Toxicity can be inferred from perturbations in high content screens observed as cell losses (cell death) or morphometric changes (e.g. cell or nuclear shape), based on the image analysis of nucl:cyto segmenting fluorescent counterstains DRAQ5™ or CyTRAK Orange™ in cell-based assays.
The far-red fluorescent viability probe DRAQ7™ can be utilized in live-cell assays to help establish levels of cellular toxicity, apoptosis induction and cell health usually combined with an “all cell” dye and/or with a mitochondrial membrane potential probe. With its ultra-low toxicity DRAQ7™ can be applied as a viability probe for real-time or long-term, low-dose exposure assays.
Recently, in vitro toxicity methods have been extended to model organisms as surrogates for human health risks, where CyGEL™ can be used to immobilize these motile objects for high-resolution imaging.
Related BioStatus Products for Flow Cytometry / Immunophenotyping
DRAQ5™ in Imaging Based in vitro Toxicity Testing
Far-red DNA counterstain, nucl:cyto segmentation in live- / fixed-end point assays.
DRAQ7™ in Imaging Based in vitro Toxicity Testing
Far-red fluorescing probe for apoptotic/dead cells in real-time cell health assays.
CyTRAK Orange™ in Imaging Based in vitro Toxicity Testing
Single-channel nucl:cyto segmentation in live- / fixed-end point assays.
CyGEL™ in Imaging Based in vitro Toxicity Testing
Convenient immobilization of motile organisms.
The study of hematotoxicity requires flow cytometry to analyse samples. The cell‐permeant DNA dyes DRAQ5™ and CyTRAK Orange™ make it easy to discriminate nucleated from enucleated cells avoiding the need for time-consuming RBC lysis, avoiding unwanted unspecific cell losses.
Stoichiometric DNA labeling with DRAQ5™ will give you information on cell cycle perturbations resulting from toxicity without the hassle of permeabilization or RNase treatment.
The far‐red viability dye DRAQ7™ allows identification or exclusion of apoptotic, damaged or dead cells, a common hallmark of toxicity.
Related BioStatus Products for Flow Cytometry Based in vitro Toxicity Testing
DRAQ5™ in Flow Cytometry Based in vitro Toxicity Testing
No-lyse, no-wash analysis of samples & Direct DNA content analysis.
DRAQ7™ in Flow Cytometry Based in vitro Toxicity Testing
Simple identification of apoptotic/dead cells in cell health analysis.
CyTRAK Orange™ in Flow Cytometry Based in vitro Toxicity Testing
No-lyse, no-wash analysis of blood/marrow samples.