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HypoxiTRAK™

HypoxiTRAK™ is a novel, far-red fluorescing dye that reveals the hypoxic experience of individual cells. It is non-toxic to normoxic cells. HypoxiTRAK™ enables entirely new assay approaches to functional hypoxia.

HypoxiTRAK™ can be used in flow cytometry and fluorescence imaging and is spectrally compatible with common visible range fluors including FITC and R-PE.

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HT10500
HypoxiTRAK™ Regular Pack
£150.00

Format

  • 10 µM, 1 × 500 µl

Typical output*

  • 50 × 1 ml Assays

Assuming a working concentration of 100 nM

Technical Information

  • HypoxiTRAK™ is supplied as a faint blue aqueous solution at a concentration of 10μM.
  • Products are shipped at ambient temperature, but on receipt packs should be stored at 2-8°C. Do NOT freeze! HypoxiTRAK™ can come out of solution when frozen and it is difficult (but not impossible) to get it back into solution.
  • HypoxiTRAK™ can be diluted in culture media (e.g. RPMI 1640) and physiological buffers (eg PBS, Hanks’s, etc.).
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You can view, share and download the information below by clicking on the links:-

HypoxiTRAK™ Technical Data Sheet (PDF)

HypoxiTRAK™ - Match your Flow Cytometer/Sorter detection channels

You can view, share and download the information below by clicking on the links:-

HypoxiTRAK™ - Preserving and Reporting the Cellular Experience of Hypoxic Environments

HypoxiTRAK™ is a NEW product from BioStatus. Check back here to find all the latest publications as they emerge.

Use our handy Google Scholar Link below then type keywords alongside the product name to find specific references – e.g. cell type, instrumentation, research focus. Google Scholar will then find all of the relevant references online.

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The far red fluorescent HypoxiTRAK™ molecular probe is designed to convert exclusively in hypoxic cells to a bioactive metabolite that retains the parent molecule fluorophore but which now accumulates in cells to provide a positive and persistent far red fluorescent signature.

  • HypoxiTRAK™ is a member of the anthraquinone family and shares spectral characteristics with the related cell permeant DNA dye DRAQ5™.
  • HypoxiTRAK™ is designed to activate by bioreduction at biologically relevant levels of hypoxic environments (e.g. tumour cores) compared to normal tissues.
  • The bioactive metabolite accumulates in cells, proportional to the hypoxia experienced, providing a persistent far-red fluorescent signature and induces cell arrest thereby marking and ‘freezing’ hypoxic cells, enabling a direct read-out of an hypoxic cell fraction.

The hypoxia sensing range for HypoxiTRAK™ is relevant to biomarker and hypoxia-targeting drug development:

  • HypoxiTRAK™ is ideal for studies over several days.
  • HypoxiTRAK™ read-out is direct with no requirement for cell processing.

As no fixation is required, HypoxiTRAK™ can provide a convenient negative selection for cells not experiencing significant hypoxia within heterogeneous populations, including 3D culture.

HypoxiTRAK™ shows low perturbation in the absence of bioactivation while the unique far-red fluorescence signature offers compatibility with other end-point assays for hypoxia that use fluorescence signatures.

in FLOW CYTOMETRY:

  • extract statistical information on the distribution of hypoxic experience over both time and treatment conditions

in FLUORESCENCE MICROSCOPY:

  • gain understanding of the spatial hypoxic experience in micro-tissues and spheroids

What our Customers are saying about HypoxiTRAK™:-

  • HypoxiTRAK™ is a NEW product from BioStatus. Be one of the first to review HypoxiTRAK™ and publish your experience here.