CyTRAK Orange™
CYTRAK Orange™ is a novel orange fluorescent dye related to DRAQ5™ that stains both nucleus and cytoplasm.
It is water soluble and membrane permeant and can be used in LIVE cells in combination with other common fluors, especially GFP fusions, FITC-labelled antibodies and far-red dyes.
CyTRAK Orange™ is available in three sizes; 50μl, 200μl and 1ml.
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CyTRAK Orange™ Regular Pack
Format
- 1 × 50 µl
Typical output*
- 250 Imaging Assays
- 100 Flow Cytometry Assays
CyTRAK Orange™ Value Pack
Format
- 1 × 200 µl
Typical output*
- 1000 Imaging Assays
- 6,600 HCS Assays
- 400 Flow Cytometry Assays
CyTRAK Orange™ Volume Pack
Format
- 1 × 1 ml
Typical output*
- 33,000 HCS Assays
Technical Information
CyTRAK Orange™ is a novel fluorescent dye that preferentially stains the nucleus, but also defines the cytoplasmic area in both LIVE and fixed preparations.
This dual compartment label provides rapid, one-step cell feature discrimination, creating new opportunities for cell-based assays where the cell location, cell perimeter, cell shape and cell spread parameters define the assay at the single cell level.
- CyTRAK Orange™ does not crosslink with or esterify cytoplasmic proteins.
- CyTRAK Orange™ does the work of two fluorophores. This leaves more opportunity and choice in designing the assay for the cells of interest.
- Products are shipped at ambient temperature, but on receipt packs should be stored at 2-8°C. Do NOT freeze! CyTRAK Orange™ can come out of solution when frozen and it is difficult (but not impossible) to get it back into solution.
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- CyTRAK Orange™ can be used as a LIVE or fixed cell counterstain or as a cell location dye to define the perimeter of the cell in a variety of cell-based assays. It can be excited at 488nm and 534nm with optimal emission at 615nm.
- It can be used in flow cytometry to separate (gate) nucleated cells without the time-consuming and potentially damaging RBC lysis procedures.
- It is highly compatible with existing protocols and proven across a wide range of instrumentation platforms: flow cytometers, epifluorescence and confocal microscopes and image-based HCS platforms.
- CyTRAK Orange™ co-excites at 488 nm with eGFP, FITC and Cy2 flurophores avoiding the need for laser alignment in confocal instruments when this combination is used.
- CyTRAK Orange™ has an emission profile that permits the simultaneous acquisition of commonly used “green” fluorphore signals (e.g. eGFP, FITC and Cy2) AND far-red FRET dyes (e.g. PE- and APC- Cy7) combined with discrimination of both the nuclear and cytoplasmic compartments.
- A separate series of parameters can be detected with red-exciting fluorphores such as APC, APC-Cy7 since CyTRAK Orange™ is not excited at 633 or 647 nm.
- Wide platform compatibility – blue/green excited (Exmax 510 nm)
- GFP/FITC compatible – orange emission (Emmax 615 nm)
- Multi-colour compatible – no UV /red excitation
- Compartments: 1 dye – differential nucl:cyto staining
- Demarks cell boundaries – even staining of the cytoplasm
- Allows assay development – works in live or fixed cells
- Time-saving – rapid staining, no-lyse/no-wash protocols
- Convenient – ready-to-use and buffer compatible
- Reliable – low photobleaching and chemically stable
What our Customers are saying about CyTRAK Orange™:-
- CyTRAK Orange™ is a novel orange fluorescent dye from BioStatus. Be one of the first to review CyTRAK Orange™ and publish your experience here.