What our customers are saying about CyTRAK Orange™
Mathis, S., et al. Leukemia 27.10 (2013): 1981-1987
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- CyTRAK Orange™ can be used as a LIVE or fixed cell counterstain or as a cell location dye to define the perimeter of the cell in a variety of cell-based assays. It can be excited at 488nm and 534nm with optimal emission at 615nm.
- It can be used in flow cytometry to separate (gate) nucleated cells without the time-consuming and potentially damaging RBC lysis procedures.
- It is highly compatible with existing protocols and proven across a wide range of instrumentation platforms: flow cytometers, epifluorescence and confocal microscopes and image-based HCS platforms.
Why CyTRAK Orange™?
- CyTRAK Orange™ co-excites at 488 nm with eGFP, FITC and Cy2 flurophores avoiding the need for laser alignment in confocal instruments when this combination is used.
- CyTRAK Orange™ has an emission profile that permits the simultaneous acquisition of commonly used "green" fluorphore signals (e.g. eGFP, FITC and Cy2) AND far-red FRET dyes (e.g. PE- and APC- Cy7) combined with discrimination of both the nuclear and cytoplasmic compartments.
- A separate series of parameters can be detected with red-exciting fluorphores such as APC, APC-Cy7 since CyTRAK Orange™ is not excited at 633 or 647 nm.
- Wide platform compatibility - blue/green excited (Exmax 510 nm)
- GFP/FITC compatible - orange emission (Emmax 615 nm)
- Multi-colour compatible - no UV /red excitation
- Compartments: 1 dye - differential nucl:cyto staining
- Demarks cell boundaries - even staining of the cytoplasm
- Allows assay development - works in live or fixed cells
- Time-saving - rapid staining, no-lyse/no-wash protocols
- Convenient - ready-to-use and buffer compatible
- Reliable - low photobleaching and chemically stable
CyTRAK Orange™ is a novel fluorescent dye that preferentially stains the nucleus, but also defines the cytoplasmic area in both LIVE and fixed preparations.
This dual compartment label provides rapid, one-step cell feature discrimination, creating new opportunities for cell-based assays where the cell location, cell perimeter, cell shape and cell spread parameters define the assay at the single cell level.
- CyTRAK Orange™ does not crosslink with or esterify cytoplasmic proteins.
- CyTRAK Orange™ does the work of two fluorophores. This leaves more opportunity and choice in designing the assay for the cells of interest.
- Products are shipped at ambient temperature, but on receipt packs should be stored at 2-8°C. Do NOT freeze! CyTRAK Orange™ can come out of solution when frozen and it is difficult (but not impossible) to get it back into solution.
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MATERIAL SAFETY DATA SHEET
What our Customers are saying about CyTRAK Orange™:-
- CyTRAK Orange™ is a novel orange fluorescent dye from BioStatus. Be one of the first to review CyTRAK Orange™ and publish your experience here.