What our customers are saying about CyGEL™
We found that CyGEL was suitable for complete immobilisation of procyclic stage L. major and T. brucei for 90 minutes without affecting cell viability. This has enabled us to analyse a GFP-tagged form of the L. major membrane-associated protein, HASPB, using live confocal imaging and FRAP. (Price et al, 2010, Molec Biochem Parasitol)
Helen Price, PhD, Keele University, UK
CyGEL™ is a thermo-reversible hydrogel. Unusually, it is a liquid when cooled and gel when warm. It is compatible with (and non-toxic to) cells, tissues and model organisms. CyGEL™ has been formulated to transition from liquid to gel at ca. 21 °C (i.e. room temperature) allowing immobilization of non-adherent (suspension) cells, beads, micro-tissues, parasites, worms and embryos (such as drosophila), which can be recovered non-destructively by cooling and/or over-dilution using cold buffer.
- CyGEL™ is optically clear, inert and non-quenching. It has no visible-range autofluorescence and a refractive index (R≃1.37) equivalent to that of water.
- CyGEL™ is designed as convenient imaging mountant and not as a growth matrix.
There are many reasons why CyGEL™ will help your research:-
IT’S EASY - ready-to-use, straight from the fridge – no powders to dissolve! IT’S GENTLE - immobilize your living cells or organisms for microscopy IT’S CLEAR - optically, it’s just like water, with no autofluorescence IT’S REVERSIBLE - image your precious sample and get it back alive!
CyGEL™ can bring significant benefits to your application:
in MICROPLATE CYTOMETRY:
- Immobilize non-adherent and motile objects for 2-D and cytometric analysis in HIGH CONTENT SCREENING:
- Immobilization of non-adherent and fragile 3-D structures in microplates in FLUORESENCE MICROSCOPY:
- Take composite images of motile organisms with confidence and control in IN VITRO TOXICOLOGY:
- Monitor cellular events and organism response in real-time and 3D in APOPTOSIS:
- Observe live non-adherent cells with in-gel probes
CyGEL™ is supplied as an aqueous solution with 40X PBS. Products are shipped at ambient temperature, but on receipt packs should be stored at 2-8 °C. Do NOT freeze! Freezing CyGEL™ may affect its performance.
CYGEL™ can be diluted 10 - 20% v/v with buffers and culture media containing cells, tissues or organisms. CyGEL™ will accept small molecules such as viability and viability dyes and anaesthetics.
You can find much more technical information in the folders below:-
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There are many independent references for CyGEL™ online. We have listed some popular papers in the link below for your convenience:
Alternatively you can use the Google Scholar facility below to find exactly what you are looking for. Just type other keywords alongside the product name - such as cell type, instrumentation, research area - in the search box below and Google Scholar will find all of the relevant references online!
SAFETY DATA SHEET
What our Customers are saying about CyGEL™:-
- "The protozoan parasites Leishmania major and Trypanosoma brucei are causative agents of leishmaniasis and African trypanosomiasis, respectively. Live imaging of these organisms is hampered by the rapid flagellum-driven motility of these species.
We found that CyGEL™ was suitable for complete immobilisation of procyclic stage L. major and T. brucei for 90 minutes without affecting cell viability. This has enabled us to analyse a GFP-tagged form of the L. major membrane-associated protein, HASPB, using live confocal imaging and FRAP. (Price et al, 2010, Molec Biochem Parasitol) ”
Helen Price, PhD, Keele University, UK
- "Under auspices of the ABRF (Association of Biomolecular Resource Facilities) there was a need to create a microscope “test” sample for imaging beyond the coverslip (100µM in Z). To accomplish this, a mounting media with a refractive index approximately that of water was needed to suspend fluorescent beads.
CyGEL™ provided a practical and cost effective solution, with excellent stability. This thermoreversible gel with its reversed viscosity/temperature properties allowed a high concentration of beads to be injected into a predetermined location with little diffusion.”
Richard Cole, PhD, SUNY, USA