Screening

High-throughput flow cytometry permits rapid sequential analysis of cell phenotypes in large sets of treated samples and controls for non-adherent cell types, blood and bone marrow.

Cell-permeant fluorescent DNA dyes can be used to discriminate nucleated from anucleated cells reducing the need for time-consuming and potentially damaging RBC lysis reagents.

Stoichiometric DNA labeling can provide information on cell cycle status, ploidy and proliferation (S-phase fraction) without any permeabilization or RNase treatment.

A fluorescent viability probe can be utilized to evaluate sample integrity, cell death or apoptosis in live cell assays.

In-Cell Western™ assays measure the change of protein expression under different conditions for a total cell population in a well. After treatment/exposure the cells are fixed and permeabilised to allow access of a fluorescently-tagged antibody to label the protein of interest.

A fluorescing stoichiometric DNA probe is normally applied to the cells in each well. The resulting signal is directly proportional to the number of cells and can be used to normalise the protein expression appropriately.