Resources Testimonials "..DRAQ5 is the most robust DNA dye.."
Testimonials description....
"We routinely perform fluorescent immunolocalization on histological sections from a wide variety of mouse tissues throughout development. Nuclear counterstaining is essential to our studies as a morphological marker, but the configuration of our confocal microscopes limits us to 488, 568 or 633 nm excitation wavelengths. We have tested nearly all DNA dyes on the market with these excitation wavelengths. We found that most either have high affinity for RNA which occludes the nuclear signal or stain the nuclei of different cell types at such wildly different brightnesses that it is impossible to image everything of interest within the dynamic range of the detector.
We find that DRAQ5 is the most robust DNA dye we have ever used. It stains nuclei much brighter than cytoplasm and allows us to obtain clear images from even the most difficult tissues such as the retina and lens. I highly recommend it!"
Melinda K. Duncan, Ph.D.
Professor and Graduate Program Director
Department of Biological Sciences
University of Delaware
Newark, Delaware, USA
Publications (citing DRAQ5):
- Production of Monoclonal Antibodies Against Prox1. Xiaoren Chen, Tapan P. Patel, William J. Cain, And Melinda K. Duncan. Hybridoma 25(1):27-33; 2006
- Conditional deletion of β1-integrin from the developing lens leads to loss of the lens epithelial phenotype. Vladimir N. Simirskii, Yan Wang, Melinda K. Duncan. Developmental Biology 306:658-668; 2007
- Deletion of JAM-A causes morphological defects in the corneal epithelium. Liang I. Kang, Yan Wang, Arthur T. Suckow, Kirk J. Czymmek, Vesselina G. Cooke, Ulhas P. Naik, Melinda K. Duncan. The International Journal of Biochemistry & Cell Biology 39:576-585; 2007
- Bin3 Deletion Causes Cataracts and Increased Susceptibility to Lymphoma during Aging. Arivudainambi Ramalingam, James B. Duhadaway, Erika Sutanto-Ward, Yan Wang, Joseph Dinchuk, Minzhou Huang, Preston S. Donover, Janette Boulden, Lois M. McNally, Alejandro P. Soler, Alexander J. Muller, Melinda K. Duncan, and George C. Prendergast. Cancer Research 68(6):1683-1690; 2008
- Differential expression of the HMGN family of chromatin proteins during ocular development. Michelle M. Lucey, Yan Wang, Michael Bustin, Melinda K. Duncan. Gene Expression Patterns 8:433-437; 2008
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