Resources Technical Documents DRAQ5 Spectral Features
Technical documents description.....
DRAQ5TM - Spectral features
- Fluorescence emission from 665 nm into the low infra-red
- Suggested optimal emission filters include 695LP, 715LP or 780 LP
- Optically compatible with benchtop flow & laser scanning cytometers and epifluorescent & non-UV confocal microscopes
- Useful excitation at sub-optimal wavelengths from the 488 nm, 568 nm and 633 nm lines, plus optimal 647 nm excitation
- No compensation needed with common FITC/GFP + PE combinations in flow cytometry
- Allows multi-line imaging/cytometry when combined with UV & vis. range fluorochromes
- Two-photon excitation demonstrated with a mode-locked laser (Nd:YLF; 1047nm) excitation
- No apparent fluorescence enhancement upon binding to DNA
- Photobleaching not normally observed
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