Resources Images Evotec Opera PC12 GFP & DRAQ5
Images description.....
PC12 cells expressing GFP tagged target protein. 3000-4000 cells in one 384 well.
Cells were fixed with PFA and stained with 20µL of 2.5µM DRAQ5™ in PBS, 30 min at RT immediately prior to image acquisition.
Image acquisition settings: standard Opera™ QEHS settings for 2 colours, 20x objective. Parallel images from the target and DRAQ5™ channels with excitations at 488 and 635nm: primary dichro 488/635, detection green: 535/60 (60), detection red: 690/50.
The green GFP fluorescence was quantified with respect to each cell (DRAQ5 stain).
Biostatus is grateful to Drs. Andreas Scheel and Stefan Jaeger, Evotec AG, Hamburg for providing these images.
DRAQ5™ GFP Overlay
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