Resources FAQs
FAQ's description....
Can I use DRAQ5, DRAQ7 or CyTRAK Orange with anti-fade mountants such as ProLong Gold, Fluoroshield, Polymount Aqua or Vectashield?
Yes.
These DNA-binding anthraquinone dyes are perfectly compatible with anti-fade mountants.
Generally, the cells are counterstained before the application of the mountant.
However, Serda, et al (2009) doped Vectashield with DRAQ5 at 5uM final concentration. Others have described the same combining DRAQ5 and ProFade Gold.
IMPORTANT: ensure that the mountant is the "native" product - DRAQ5 cannot be combined with mountants containing DAPI as the DAPI quenches the DRAQ5 staining signal.
- Email: enquiry@biostatus.com
- Telephone: +44 1509 558163
- Fax: +44 1509 651061