DRAQ7™ NEW!! spectral profile
The graphs below show standard absorbance and fluorescence emission spectra for DRAQ7™ in aqueous solution for a range of different excitation wavelengths. As can be seen, DRAQ7™ can be effectively excited by 488nm systems. In these circumstances, we recommend that a long band pass filter be used - to capture as many emitted photons as possible.
Excitation:
633 & 647 nm line optimal (Exλmax 599/644 nm)
488, 514 and 568 lines, sub-optimal (only by flow cytometry)
Emission:
>665 nm to infra-red >800 nm (Emλmax 678 nm / 694 nm intercalated with dsDNA)
Minimal overlap with vis range e.g. GFP and FITC
Emission filters may include 695LP, 715LP or 780 LP
Multi-wavelength imaging with UV / vis fluorochromes
No fluorescence enhance-ment upon DNA binding
Low photobleaching effect
Compatible with optics of flow, laser scanning cytometers and confocal and lamp-based fluorescence microscopes
- Email: enquiry@biostatus.com
- Telephone: +44 1509 558163
- Fax: +44 1509 651061