A new live-cell impermeant, far-red emitting DNA dye ..
key applications for DRAQ7 |
FLOW CYTOMETRY |
apoptosis assays |
- labels ONLY leaky/dead cells
- enables multi-colour assays
| - convenient FAR-RED replacement for PI and 7-AAD
- opens up PE channel
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dead cell gating / sample quality
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- enumerates dead cells for exclusion / sample integrity
- directly interchangeable with DRAQ5 (for DNA content)
| - more parameters from blue laser
- powerful 2-tube analysis: cell cycle (DRAQ5) & sample cellular integrity (DRAQ7)
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combination tracking of cell health / status
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- combine with live cell dyes
| - FAR-RED emission allows for more parameters than PI
|
real time, kinetic viability assays |
| | - FAR-RED emission allows for more parameters than PI
|
MICROSCOPY & IMAGING
| |
cell-based HCS assays (e.g. RNA interference / compound screening)
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- avoids UV light exposure
- enters ONLY dead / leaky cells
- emission profile allows single pass scanning (halves time required)
| - vis. range excitation - no UV "white out" of 10-15% of library members
- GFP-tags and cells located in one plate scan for higher throughput
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fixed cell assays / imaging / FISH
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- stains nucleus & cytoplasm differentially
- spectrally compatible with all visible range fluors
| - streamlines assay development
- fixed cell/tissue alternative to DAPI
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