A new live-cell impermeant, far-red emitting DNA dye ..
features | benefits | links | spectral- visible range excitation (blue or red)
- far-red emission
- non-enhanced DNA dye
| - can be used on a wide range of instrument platforms
- no overlap with GFP/FITC or PE therefore no need to compensate
- no need to wash out
| read more | binding/labeling- DNA specific
- membrane-impermeant
- can see nuclear architecture
| - nucl:cyto visualization
- DEAD cells and FIXED cells
- can use in most cell types - mammalian, bacterial, parasitic, plant etc...
| read more | chemical- buffer compatible / water soluble
- low photobleaching
- membrane-impermeant
| - no-wash protocols
- very rapid staining in DEAD and FIXED cells
- DOES NOT LABEL INTACT LIVE CELLS
- conveniently supplied in aqueous solution
| read more | flow cytometry- "viability gate"
- spectrally separated
- ideal for FITC/PE pairs
- combines with "live" dyes
| - many applications in research, clinical and industrial settings
- multiple excitation allows laser alignment on cells
- DEAD CELL exclusion
- apoptosis / leaky membrane indicator (in far-red)
| read more | imaging and laser scanning cytometry | - nuclear "gate" or "trigger" for fixed cell samples.
- DEAD cell / apoptosis "gate"
- flexible protocol development
| read more | live cell imaging- Rapid staining of dead / membrane-leaky cells
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- for cytotoxicity assays
- apoptosis assays
| read more | cell-based assays (fixed,end-point)
- avoids UV excitation
- reliable staining
- allows single pass scanning
| - nucl:cyto differential staining ideal for translocation assays
- halve scan times cf. Hoechst / DAPI
- no UV "white out" - 10-15% more efficient screening of library compounds
| read more |
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