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CNIO images Pathway

DRAQ7™ NEW!!

A new live-cell impermeant, far-red emitting DNA dye ..

  • DEAD cell exclusion in flow cytometry
  • DEAD / APOPTOTIC cell labeling
  • FIXED cell labeling in HCS / IF / IHC 
  • NEW combinations in cell health / status assays

What is it?

DRAQ7™ is a new far-red fluorescent DNA dye that ONLY stains the nuclei in DEAD and permeabilized cells.  It can be combined with other common fluors, especially GFP fusions & FITC and PE labeled antibodies.

It can be excited by the blue, green or red light in a flow cytometer and red light in imaging instruments. 

DEAD CELLS / MEMBRANE-COMPROMISED CELLS:

DRAQ7™ does NOT enter intact, live cells. It is excited by blue through red lasers. 

DRAQ7™ is NON-TOXIC in long-term culture (see example in SU-DHL4 cells)

DRAQ7™ is an ideal replacement for propidium iodide (and 7-AAD) having far better spectral properties: no UV excitation and no emission overlap with PE and homologues.

DRAQ7™ allows a new design of combination analysis for cell health and status.

DRAQ7™ (viability/integrity) can be directly interchanged with DRAQ5™ (DNA content)

FIXED CELLS & TISSUE SECTIONS:

DRAQ7™ is an ideal far-red DNA counterstain for IF/IHC, high content screening and cell-based assays

What are the key benefits?

DRAQ7™ has many applications and is highly compatible with existing protocols across a wide range of instrumentation platforms.

Key benefits include:-

  • Rapid staining of dsDNA/nuclei of DEAD or permeabilized cells
  • Combination with live cell dyes for dead/live discrimination
  • Easy to use - no wash, no RNase needed
  • Ideal for use with GFP & FITC labels - DRAQ7 fluoresces in the far-red region
  • Cost-effective analysis

Where can I buy DRAQ7™?

DRAQ7™ can be purchased from this website. See our purchase pages for full details.

Links to further information

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