DRAQ5™ spectral profile
A novel LIVE cell DNA dye that emits in the far-red...
The graph below shows standard fluorescence emission spectra for DRAQ5™ in aqueous solution for a range of different excitation wavelengths. As can be seen, DRAQ5™ can be effectively excited by 488nm systems. In these circumstances, we recommend that a long band pass filter be used - to capture as many emitted photons as possible.
Excitation:
- 647 nm line optimal (Exλmax 646 nm)
- 488, 514, 568 and 633 nm lines, sub-optimal
- Two-photon excitation (1047 nm) and excitation dark (700-850 nm)
Emission:
- >665 nm to infra-red >800 nm (Emλmax 681 nm / 697 nm intercalated with dsDNA)
- Minimal overlap with vis range e.g. GFP and FITC
- Em. filters may include 695LP, 715LP or 780 LP
Multi-wavelength imaging with UV / vis fluorochromes
- No fluorescence enhancement upon DNA binding
- No photobleaching effect
- Compatible with optics of benchtop flow, laser scanning cytometers and non-UV laser scanning and lamp-based confocal microscopes
- Email: enquiry@biostatus.com
- Telephone: +44 1509 558163
- Fax: +44 1509 651061