DRAQ5™ features and benefits
A novel LIVE cell DNA dye that emits in the far-red...
features | benefits | links |
spectral- visible excitation
- far-red emission
- no appreciable auto-fluorescence
| - can be used on a wide range of instrument platforms
- no overlap with GFP/FITC therefore no need to compensate
- no need to wash out
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binding/labeling- DNA specific
- stoichiometric
- can see nuclear architecture
| - nucl:cyto visualisation
- cell cycle analysis
- LIVE cells and FIXED cells
- can use in most cell types - mammalian, bacterial, parasitic, plant etc...
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chemical- buffer compatible
- low photobleaching
- membrane-permeant
| - no lyse / no wash protocols
- very rapid staining in LIVE and FIXED cells
- conveniently supplied in aqueous solution
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flow cytometry- easy cell-cycle method
- "nuclear gate"
- ideal for FITC/PE
| - many applications in research, clinical and industrial settings
- multiple excitation allows laser alignment on cells
- LIVE or FIXED cells
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flow and laser scanning cytometry | - clinical protocols being developed that allow for whole blood discrimination with small sample volumes
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live cell imaging | - no UV exposure
- see nuclear morphology and architecture
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cell-based assays- avoids UV excitation
- stains EVERY cell in the well
- allows single pass scanning
| - nucl:cyto differential staining ideal for translocation assays
- halve scan times cf. Hoechst / DAPI
- no UV "white out" - 10-15% more efficient screening of library compounds
- include MDR cells
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