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Mouse Embryo

CyTRAK Orange™ key applications

A novel orange fluorescing nuclear/cytoplasm dye for live cells ....

key applications for CyTRAK Orange

short-term live cell imaging

 
  • nucleus/cytoplasmic membrane translocations
  • nucleus/cytoplasm translocations
  • cytoplasm/cytoplasmic membrane translocations
  • cell/cell boundary interactions
  • see the nucleus and cytoplasm as separate compartments to follow any GFP-tagged temporospatial change in a live cells in a single acquisition from a 488 nm excitation
  • scan a far-red parameter (e.g. Mitotracker Far Red) by serial excitation at 633 nm

cell-based HCS assays

 
  • no need to use UV excitation
  • will enter EVERY cell in the well
  • emission profile allows single pass scanning
  • not actively pumped
  • view the whole cytoplasmic field
  • vis. range excitation - no UV "white out" of 10-15% of library compounds
  • GFP-tags and cells located in one plate scan for higher throughput
  • include MDR cells

fixed cell assays / imaging / FISH

 
  • stains nucleus and cytoplasm differentially
  • spectrally compatible with green (e.g. FITC) and far-red (Cy5) fluorescent probes
  •  image cytoplasmic (e.g. GFP) and nuclear (CyTRAK Orange) markers co-incidentally

 flow cytometry

 
  • gate nucleated cells from debris in difficult samples: de-adhered cells / tissue digests and in rare event detection
  • blue laser co-excitation with FITC/GFP
  • No UV or red excitation problems