A novel orange fluorescing nuclear/cytoplasm dye for live cells ....
key applications for CyTRAK Orange |
real-time live cell imaging | |
- nucleus/cytoplasmic membrane translocations
- nucleus/cytoplasm translocations
- cytoplasm/cytoplasmic membrane translocations
- cell/cell boundary interactions
| - see the nucleus and cytoplasm as separate compartments to follow any GFP-tagged temporospatial change in a live cells in a single acquisition from a 488 nm excitation
- scan a far-red parameter (e.g. Mitotracker Far Red) by serial excitation at 633 nm
|
cell-based HCS assays | |
- no need to use UV excitation
- will enter EVERY cell in the well
- emission profile allows single pass scanning
- not actively pumped
| - view the whole cytoplasmic field
- vis. range excitation - no UV "white out" of 10-15% of library compounds
- GFP-tags and cells located in one plate scan for higher throughput
- include MDR cells
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fixed cell assays / imaging / FISH
| |
- stains nucleus and cytoplasm differentially
- spectrally compatible with green (e.g. FITC) and far-red (Cy5) fluorescent probes
| - image cytoplasmic and nuclear markers co-incidentally
|