A novel orange fluorescing nuclear/cytoplasm dye for live cells ....
features | benefits | links |
spectral | | |
- low visible range (457, 488, & 549 nm) excitation
- No excitation by UV /red lasers
- orange emission
- no emission overlap with co-excited "green" fluors
- no appreciable auto-fluorescence
| - can be used on a wide range of imaging platforms
- single scan with GFP/FITC/Cy2 ...
- ... then, dual scan with red excited dyes for more parameters
- no need to wash out
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binding/labeling | | |
- differential intensity DNA binding & Cytoplasmic labelling
- non-crosslinking
- One fluorophore doing the work of two
| - nuclear / cytoplasm locator
- see nuclear and cytoplasmic boundaries
- for LIVE and FIXED cells
- useful in most cell types
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chemical | | |
- assay buffer compatible
- low photobleaching
- membrane-permeant
- No DMSO required
- Store in fridge (2-8ºC)
| - no lyse / no wash protocols
- easy to use
- rapid staining
- convenient aqueous solution
- Real-time temporospatial event imaging in LIVE cells
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