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Drosphila Embryo

CyGEL Sustain™ temperature and viscosity

Sol-gel Transition Temperature and Viscosity

The graphs below, show the relationship between sol-gel transition temperature and viscosity for CyGEL™ over a range of concentrations. Please note that this data has been generated using CyGEL™ in aqueous solution. CyGEL Sustain is designed to be used with RPMI or RPMI-like culture media for LIVE cell analyses, with similar viscosity, but convenient gel formation and immobilisation properties at around 23°C.

CyGEL gelation visco combo

The basic sol-gel transition temperature and viscosity of CyGEL™ can be modified by the addition of a variety of additives (see table below). CyGEL™ is provided at 100% strength. If you have an interest in using a CyGEL formulation for a specific application, please contact us.

 

 

Additive:

Cygel transition temperature change

(°C)

CyGel viscosity change at 37°C

(Pa s)

1% w/v sorbitol

-1.4

+40

5% w/v sorbitol

-3.0

-2

1% w/v hydroxyethylcellulose

-0.3

+397

5% w/v hydroxyethylcellulose

-2.2

+829

1% w/v glycerol

-0.2

-53

5% w/v glycerol

-2.4

+195

1% w/v sodium chloride

-4.2

+286

5% w/v sodium chloride

-10.3

+498

1% w/v propylene glycol

-0.8

+283

5% w/v propylene glycol

-3.4

+383

1% w/v polyethylene glycol 400

0.0

+75

5% w/v polyethylene glycol 400

+0.2

+96

1% w/v polyethylene glycol 2000

+1.1

+386

5% w/v polyethylene glycol 2000

+2.8

+651