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• APOPTRAK™
  • general overview
  • material safety data sheet (msds)
  • tracking apoptosis by flow cytometry
  • example protocol
  • key reference
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• DRAQ5™
  • general overview
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  • spectral profile
  • live cell comparison chart
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• CyTRAK Orange™
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  • high-content cell assays
  • imaging with egfp (example)
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• CyGEL™
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  • easy mounting of cells
  • immobilisation in practice
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  • long-term imaging live non-adhered cells
  • controlled delivery of fluorochromes
  • draq5 fluorescence in cygel
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  • apoptosing cells in pi/cygel
  • temperature and viscosity
  • refractive index and temperature
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• CyGEL Sustain™
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  • easy mounting of cells
  • immobilisation in practice
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  • long-term imaging live non-adhered cells
  • controlled delivery of fluorochromes
  • draq5 fluorescence in cygel
  • cells in draq5/cygel
  • apoptosing cells in pi/cygel
  • temperature and viscosity
  • refractive index and temperature

Applications

• HTS / HCS Screening
  • general overview
  • nuclear visualisation
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  • Evotec Opera™ assays
  • BD Pathway 855™ Assays
  • microplate cytometry
  • LiCor InCell Western Assay
  • siRNA induced apoptosis assays
• Confocal Microscopy
  • general overview
  • live cell imaging
  • live cell mountant
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  • fixed cells / nuclei
  • plant tissue
• Flow Cytometry
  • general overview
  • whole blood differential
  • bone marrow differential
  • cell cycle analysis
  • cell ploidy / aneuploidy
  • apoptosis detection
  • detecting CD45neg cells
  • laser alignment
  • residual leukocyte detection
  • platelet / megakaryocyte differentiation
  • RBC malarial parasite detection
• Laser Scanning Slide & Microplate Cytometry
  • general overview
  • cell cycle analysis on Acumen eX3
  • cell cycle analysis on LSC
  • whole blood differential
• Lamp/Camera Microscopy
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  • FISH
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• MicroArray and Biochip technologies

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CyGEL Sustain™ refractive index and temperature

Refractive Index and Temperature

The graph opposite, shows the relationship between refractive index and temperature for a range of CyGEL™ concentrations.

Please note that this data has been generated using CyGEL™ in aqueous solution.

CyGEL Sustain is designed to be used with RPMI and RPMI-like culture media for LIVE cell analyses, with similar viscosity, but convenient gel formation and immobilisation properties at around 23°C.

CyGEL™ is provided at 100% strength. If you have an interest in a CyGEL formulation for a specific application, please contact us.