CyGEL Sustain™
What is it?
CyGEL Sustain is a novel thermoreversible gel that is compatible with extended imaging of LIVE cells, for several hours depending on cell type.
Its special formulation is optimised for the addition of RPMI and similar culture media.
It can be used to immobilise non-adherent cells and beads by simple warming, and conversely allowing their recovery by simple cooling. CyGEL Sustain is unusual in that it is a liquid when cold and a gel when warmed!
What are the key benefits?
CyGEL Sustain has many applications in imaging, flow cytometry and cell-based screening. It is compatible with LIVE cells, usefully allowing their immobilisation for analysis and subsequent release for ongoing experimentation.
Key benefits include:-
- optically clear with low autofluorescence
- controllable and rapid transition from liquid to gel
- convenient immobilisation of non-adherent cells and beads
- compatible with GFP and "in gel" fluorescent probes including DRAQ5™
Advantages:
- allows rapid preparation of live cells for gel mounting
- permits long term analysis of live non-adherent cells
- acts as a support matrix
- permits sterile, low temperature recovery of cells from gel
- multiple assay formats and applications including apoptosis detection
Where can I buy CyGEL Sustain?
CyGEL Sustain can be purchased from this website. See our purchase pages for full details.
Links to further information
- Features and Benefits
- key references
- easy mounting of cells
- immobilisation in practice
- protocols
- long-term imaging LIVE non-adhered cells
- controlled delivery of fluorochromes
- DRAQ5 fluorescence in CyGEL
- cells in DRAQ5/CyGEL
- apoptosing cells in PI/CyGEL
- temperature and viscosity
- refractive index and temperature
| Most Recent Related Resources | Type |
|---|---|
| CyGEL Sustain Protocols | Technical Documents |
- Email: enquiry@biostatus.com
- Telephone: +44 1509 558163
- Fax: +44 1509 651061