CyGEL Sustain™
What is it?
CyGEL Sustain is a novel thermoreversible gel that is compatible with extended imaging of LIVE cells and Zebrafish embryos for several hours depending on cell/organism type.
Its special formulation is optimised for the addition of RPMI and similar culture media.
It can be used to immobilise non-adherent cells and beads by simple warming, and conversely allowing their recovery by simple cooling. CyGEL Sustain is unusual in that it is a liquid when cold and a gel when warmed!
(n.b. For live C. elegans and parasites imaging we recommend CyGEL)
What are the key benefits?
CyGEL Sustain has many applications in imaging, flow cytometry and cell-based screening. It is compatible with LIVE cells, usefully allowing their immobilisation for analysis and subsequent release for ongoing experimentation.
Key benefits include:-
- optically clear with low autofluorescence
- controllable and rapid transition from liquid to gel
- convenient immobilisation of non-adherent cells and beads
- compatible with GFP and "in gel" fluorescent probes including DRAQ5™
Advantages:
- allows rapid preparation of live cells, zebrafish for gel mounting
- permits long term analysis of live non-adherent cells
- acts as a support matrix
- permits sterile, low temperature recovery of cells from gel
- multiple assay formats and applications including apoptosis detection
Where can I buy CyGEL Sustain?
CyGEL Sustain can be purchased from this website. See our purchase pages for full details.
Links to further information
- Features and Benefits
- LIVE zebrafish embryo imaging
- key references
- easy mounting of cells
- immobilisation in practice
- protocols
- long-term imaging LIVE non-adhered cells
- controlled delivery of fluorochromes
- DRAQ5 fluorescence in CyGEL
- cells in DRAQ5/CyGEL
- apoptosing cells in PI/CyGEL
- temperature and viscosity
- refractive index and temperature
- Material Safety Data Sheet
- Email: enquiry@biostatus.com
- Telephone: +44 1509 558163
- Fax: +44 1509 651061