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Mouse Embryo

CyGEL™ long-term imaging LIVE non-adhered cells

CyGEL™ uniquely enables imaging of LIVE non-adhered cells over several hours ..

The recently published work of Upton, et al from the laboratory of Andrew P Gilmore (Manchester, UK) used CyGEL™ to immobilise epithelial cells after detachment from the extra-cellular matrix (ECM).

The features of CyGEL™ were key since:

  • The stochastic apoptotic processes required several hours of imaging
  • The cells had to be kept away from surfaces such as the imaging chamber coverslip and from potential cell-cell contact
  • The chosen cells required an environment of supporting medium and growth factors for continued viability
  • Fluorescence imaging required an optically-inert, clear scaffold for the cells

See / download the technical document reviewing the use of CyGEL™ in this work