CyGEL™ Live Parasite Imaging
- CyGEL™ allows complete immobilisation of LIVE parasites
- Organisms can be recovered live after up to 2 hours
- CyGEL™ accepts fluorescent viability and functional probes
CyGEL™ has been validated for imaging live parasites, demonstrated with Trypanosoma sp. and Leishmania sp.
- quicker and simpler to use, better reproducibility than agarose. Optically inert and clear.
- imaging for up to 2 hours with good viability and recovered alive!
- compatible with fluorescent viability (PI) and organelle-specific (FM4-64, LysoTracker, Mitotracker) probes.
- complete immobilisation allowing full breadth of advanced microscopic techniques (including FRAP used by authors below).
Reference:
Helen P. Price, Lorna MacLean, Joanne Marrison, Peter J. O’Toole, Deborah F. Smith,Validation of a New Method for Immobilising Kinetoplastid Parasites for Live Cell Imaging, Molecular and Biochemical Parasitology (2009), doi:10.1016/j.molbiopara.2009.09.008
Image: Leishmania major; transfected with HASPB-GFP, stained with LysoTracker Red DND-99 mounted in CyGEL™ for 90 minutes when imaged.
Image courtesy of Dr Lorna MacLean, Centre for Immunology and Infection, Department of Biology / Hull York Medical School, University of York, UK
- Email: enquiry@biostatus.com
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