News DRAQ5 in new blood & BM differential method
DRAQ5 in new blood & BM differential method
Posted: 25-Apr-2008
A powerful and simple new methodology for a total nucleated cell differential has been recently published in the Journal Cytometry B (Clinical Cytometry).
This new method uses DRAQ5 to gate nucleated cells, a panel of 6 antibodies in 4 groups and the 5 fluorescence channels of the Beckman Coulter FC500.
This one-tube, lyse-no wash method shows excellent correlation to cell counters and microscopy and offers a real alternative to the laborious manual scoring of smears from samples flagged by the cell counter.
Using the DNA binding properties of DRAQ5 cell cycle information was also available.
Using the DRAQ5-, CD36+ events it was also possible to enumerate the platelets.
View the abstract and the full article using the link below:
Bjornsson et al. Total nucleated cell differential for blood and bone marrow using a single tube in a five-color flow cytometer. Cytometry B 2008; 74B: 91-103
You can download literature reviews on this application area for DRAQ5 by linking here: total nucleated cell differential, for a recent alternative strategy for bone marrow aspirate evaluation, or cell cycle analysis of blood / BM
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