Immunofluorescence Microscopy (i.e. fixed cells or tissue sections)
![](/site/biostatus/images/pages-applications/Immunofluorescence microscopy app.png)
Immunofluorescence involves microscopical analysis of cells and paraffin-embedded or frozen tissue sections. Samples are fixed (usually with formaldehyde) to preserve the phenotype and permeabilized to allow fluorescently-labelled antibodies access to the internal antigens. Use of a fluorescent DNA probe allows counterstaining and therefore location of individual cells, segmentation of their nuclei and total cell enumeration.
Non-adherent cells, micro-tissues or motile organisms may require immobilisation to permit reliable and high-performance imaging of structures and changes.
Related BioStatus Products for Immunofluorescence Microscopy
Click on the orange headings to view the Application Notes
![](/site/biostatus/images/pages-applications/DRAQ5 immunofluorescence microscopy app.png)
DRAQ5™ in Immunofluorescence Microscopy
Far-red fluorescing DNA counterstain; GFP/FITC compatible, no UV needed.
VIEW PRODUCT![](/site/biostatus/images/pages-applications/CyTRAK Orange immunofluorescence microscopy app.png)
CyTRAK Orange™ in Immunofluorescence Microscopy
Orange fluorescing DNA counterstain; GFP/FITC compatible, no UV needed.
VIEW PRODUCT![](/site/biostatus/images/pages-applications/CyGEL immunofluorescence microscopy app.png)
CyGEL™ in Immunofluorescence Microscopy
Convenient immobilization of non‐adherent or fragile objects
VIEW PRODUCT