Flow Cytometry detecting CD45neg cells
reliable partner in clinical analysis...
DRAQ5™ can be used retrospectively to label nucleated CD45neg cells and segregate their analysis from anucleated cells
CD45 negative nucleated cells in a sample may come from two expected sources:
- nucleated RBCs i.e. erythroid precursors - reticulocytes
- nucleated platelet precursors - megakaryocytes
and also from occult sources e.g. endothelial cells, postulated to come from venous damage or non-haematological tumour cells e.g. circulating carcinoma derived cells
Label samples with DRAQ5™ to establish which cells are CD45 nucleated and anucleated using the standard protocol. DRAQ5™ positive cells will be detected using a far-red filter e.g. Cy5 or preferably 715LP. If it is intended to establish cell cycle status or ploidy for these cells then DRAQ5™ concentration should be >10µM and nucleated cell density should be ≤ 0.4 x 10 6 / ml.
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