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BioStatus Limited
56A Charnwood Road
Shepshed
Leicestershire
LE12 9NP
United Kingdom

Tel: +44 1509 558 163
Fax: +44 1509 651 061

Email: enquiry@biostatus.com

Company No 3079239
VAT# GB 755 5893 80

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CyGEL™

CyGEL™ is a novel thermoreversible gel that is compatible with LIVE cells and organisms. It can be used to immobilise non-adherent cells and beads by simple warming and conversely allowing their recovery by simple cooling.

CyGEL™ is unusual in that it is a liquid when cold and a gel when warmed!

CLICK HERE to view applications suitable for CyGEL™

CyGEL™ is available in two sizes; a 8 x 500μl pack which includes 1 x 125μl PBS(40X), or a 20ml bulk value pack for large volume use. You can order CyGEL™ directly from this website - see below:

SKU DESCRIPTION PRICE QTY
  • CY10500
  • CyGEL™
    Regular Pack
    8x 500µl
    1x 125µl PBS(40x)
    Immobilisation Medium
  • Price: £140.00 (Excl. VAT)
  •  
  • CY20000
  • CyGEL™
    Volume Pack
    1x 20ml
    625 imaging assays
  • Price: £700.00 (Excl. VAT)
  •  

Product Reviews

What our customers are saying about CyGEL™

 The protozoan parasites Leishmania major and Trypanosoma brucei are causative agents of leishmaniasis and African trypanosomiasis, respectively. Live imaging of these organisms is hampered by the rapid flagellum-driven motility of these species.

We found that CyGEL was suitable for complete immobilisation of procyclic stage L. major and T. brucei for 90 minutes without affecting cell viability. This has enabled us to analyse a GFP-tagged form of the L. major membrane-associated protein, HASPB, using live confocal imaging and FRAP. (Price et al, 2010, Molec Biochem Parasitol)  

Helen Price, PhD, Keele University, UK

The Science

CyGEL™ is a thermo-reversible hydrogel. Unusually, it is a liquid when cooled and gel when warm. It is compatible with (and non-toxic to) cells, tissues and model organisms. CyGEL™ has been formulated to transition from liquid to gel at ca. 21 °C (i.e. room temperature) allowing immobilization of non-adherent (suspension) cells, beads, micro-tissues, parasites, worms and embryos (such as drosophila), which can be recovered non-destructively by cooling and/or over-dilution using cold buffer.

  • CyGEL™ is optically clear, inert and non-quenching. It has no visible-range autofluorescence and a refractive index (R≃1.37) equivalent to that of water.
  • CyGEL™ is designed as convenient imaging mountant and not as a growth matrix.

Why CyGEL™?

There are many reasons why CyGEL™ will help your research:-

IT’S EASY - ready-to-use, straight from the fridge – no powders to dissolve!
IT’S GENTLE - immobilize your living cells or organisms for microscopy
IT’S CLEAR - optically, it’s just like water, with no autofluorescence
IT’S REVERSIBLE - image your precious sample and get it back alive!

CyGEL™ can bring significant benefits to your application:

    in MICROPLATE CYTOMETRY:
  • Immobilize non-adherent and motile objects for 2-D and cytometric analysis
  • in HIGH CONTENT SCREENING:
  • Immobilization of non-adherent and fragile 3-D structures in microplates
  • in FLUORESENCE MICROSCOPY:
  • Take composite images of motile organisms with confidence and control
  • in IN VITRO TOXICOLOGY:
  • Monitor cellular events and organism response in real-time and 3D
  • in APOPTOSIS:
  • Observe live non-adherent cells with in-gel probes

Technical Information

CyGEL™ is supplied as an aqueous solution with 40X PBS. Products are shipped at ambient temperature, but on receipt packs should be stored at 2-8 °C. Do NOT freeze! Freezing CyGEL™ may affect its performance.

CYGEL™ can be diluted 10 - 20% v/v with buffers and culture media containing cells, tissues or organisms. CyGEL™ will accept small molecules such as viability and viability dyes and anaesthetics.

You can find much more technical information in the folders below:-

KEY REFERENCES

Search Online:


There are many independent references for CyGEL™ online. We have listed some popular papers in the link below for your convenience:

CyGEL™ Popular References (PDF)

Alternatively you can use the Google Scholar facility below to find exactly what you are looking for. Just type other keywords alongside the product name - such as cell type, instrumentation, research area - in the search box below and Google Scholar will find all of the relevant references online!

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SAFETY DATA SHEET

CyGEL™ SDS


You can view, share and download a country specific Safety Data Sheet (PDF) in your language by clicking the relevant flag below:-

Hover over the flags to reveal the Country and Language:-

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CUSTOMER REVIEWS

CyGEL™ - Reviews


What our Customers are saying about CyGEL™:-

  • "The protozoan parasites Leishmania major and Trypanosoma brucei are causative agents of leishmaniasis and African trypanosomiasis, respectively. Live imaging of these organisms is hampered by the rapid flagellum-driven motility of these species.
    We found that CyGEL™ was suitable for complete immobilisation of procyclic stage L. major and T. brucei for 90 minutes without affecting cell viability. This has enabled us to analyse a GFP-tagged form of the L. major membrane-associated protein, HASPB, using live confocal imaging and FRAP. (Price et al, 2010, Molec Biochem Parasitol) ”
    Helen Price, PhD, Keele University, UK

  • "Under auspices of the ABRF (Association of Biomolecular Resource Facilities) there was a need to create a microscope “test” sample for imaging beyond the coverslip (100µM in Z). To accomplish this, a mounting media with a refractive index approximately that of water was needed to suspend fluorescent beads.
    CyGEL™ provided a practical and cost effective solution, with excellent stability. This thermoreversible gel with its reversed viscosity/temperature properties allowed a high concentration of beads to be injected into a predetermined location with little diffusion.”
    Richard Cole, PhD, SUNY, USA