Image-based screening is essentially automated fluorescence microscopy performed on large numbers of samples, commonly of adherent cells in wells of micro plates. Use of a fluorescent DNA probe allows counterstaining and therefore location of individual cells, segmentation of their nuclei and total cell enumeration. A cell-permeant counterstain permits choice of either live- or fixed-cell endpoint assay and simple transfer between the two.
A fluorescent viability probe can be utilized in live-cell assays to help establish levels of cellular toxicity, apoptosis induction and cell health.
Non-adherent cells or microtissues or motile organisms may require immobilisation to permit reliable and high-performance imaging of structures and changes.
Related BioStatus Products for Image-Based Screening
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DRAQ5™ in Image-Based Screening
Far-red DNA counterstain, nucl:cyto segmentation in live- / fixed-end point assays.VIEW PRODUCT
DRAQ7™ in Image-Based Screening
Reliable identification of apoptotic/dead cells in real-time.VIEW PRODUCT
CyTRAK Orange™ in Image-Based Screening
Single-channel nucl:cyto segmentation in live- / fixed-end point assays.VIEW PRODUCT
CyGEL™ in Image-Based Screening
Convenient immobilization of live non-adherent / motile objects.VIEW PRODUCT
CyGEL Sustain™ in Image-Based Screening
Culture medium-specific immobilization of live non-adherent / motile objects.VIEW PRODUCT