Imaging-Based Cell Health Assays
The most common image-based assays for cell health combine total cells (e.g. with Hoechst 33342), mitochondrial health (TMRM) and plasma membrane integrity. DRAQ7™ is the ideal viability probe for plasma membrane integrity due to its far-red fluorescence, sensitivity, ready-to-use formulation and cost-effectiveness.
Uniquely, due to its validated ultra-low toxicity DRAQ7™ can be used in real-time, long-term cell health assays.
In more sophisticated assays a cell permeant nuclear counterstain can be combined with other parameters. Using the far-red, cell permeant DNA probe DRAQ5™ one can get cell numbers, nuclear condensation, nuclear and cytoplasmic morphology and DNA content / cell cycle position.